Category: Application Notes

Reagents for Antisense Oligonucleotide (ASO) Delivery Application Note: Use of RJH Transfection Reagents in Antisense Oligonucleotide (ASO) Delivery BACKGROUND The convergences of sophisticated delivery systems, oligonucleotide chemistry and genetic bioinformatics has significantly enhanced the exploration of antisense oligonucleotides (ASO) for therapeutic purposes, which has been reflected in many clinical studies (Nat. Rev. Drug Discov. 11,… Continue reading Reagents for Antisense Oligonucleotide (ASO) Delivery

Comparing Lipofection to RJH Reagents Application Note: Comparison of Transfection Reagents for pDNA and mRNA Delivery BACKGROUND Peripheral blood derived mononuclear cells (PBMCs) are important mediators involved in immune surveillance and are actively explored as the foundation of a wide range of therapies [1]. It is convenient to isolate the cells from patients and re-administer… Continue reading Comparing Lipofection to RJH Reagents

mRNA Modification of PBMCs Application Note: Use of RJH Transfection Reagents for mRNA Delivery to PBMCs BACKGROUND Peripheral blood derived mononuclear cells (PBMCs) are important mediators involved in immune surveillance and are actively explored as the foundation of a wide range of therapies [1]. It is convenient to isolate the cells from patients and re-administer… Continue reading mRNA Modification of PBMCs

mRNA-Fect Transfection Reagent to Deliver mRNA in Breast Cancer Cells Application Note: mRNA-Fect Transfection Reagent to Deliver mRNA in Breast Cancer Cells BACKGROUND Messenger RNA (mRNA) therapy has been developed as an alternative to DNA-based therapies as it avoids several limiting issues associated with the DNA delivery. It can efficiently modify hard-to-transfect non-dividing cells since… Continue reading mRNA-Fect Transfection Reagent to Deliver mRNA in Breast Cancer Cells

Transfecting Triple-Negative Breast Cancer MDA-MB-231 Cells with Plasmid DNA and siRNA by using ALL-Fect and Prime-Fect Application Note: Transfecting Triple-Negative Breast Cancer MDA-MB-231 Cells with Plasmid DNA and siRNA by using ALL-Fect and Prime-Fect Phase Contrast (top) and Fluorescence Microscopy (bottom) pictures of breast cancer cells transfected with a leading polymeric reagent and ALL-Fect. A leading… Continue reading Transfecting Triple-Negative Breast Cancer MDA-MB-231 Cells with Plasmid DNA and siRNA by using ALL-Fect and Prime-Fect

Use of RJH Transfection Reagents in Co-Delivery of Plasmid DNA and short interfering RNA Application Note: Use of RJH Transfection Reagents in Co-Delivery of Plasmid DNA and short interfering RNA BACKGROUND The convergence genetic bioinformatics and nucleic acid (DNA and RNA) chemistry with sophisticated delivery systems has significantly enhanced therapeutic application of nucleic acids. The… Continue reading Use of RJH Transfection Reagents in Co-Delivery of Plasmid DNA and short interfering RNA

RJH Transfection Reagents in siRNA Library Screens Application Note: RJH Transfection Reagents in siRNA Library Screens BACKGROUND The structured oligonucleotide libraries allow one to screen large number of compounds for specific purposes. In biomarker discovery, library screens facilitate identification of critical mediators that are responsible for pathological conditions. In therapeutic discovery, library screens allow one… Continue reading RJH Transfection Reagents in siRNA Library Screens

microRNA Delivery to Leukemic Cells Use of RJH Transfection Reagents to Deliver microRNAs into Leukemic Cells BACKGROUND The convergence of genetic bioinformatics and nucleic acid chemistry along with sophisticated transfection systems have significantly expanded scientific and therapeutic applications of nucleic acids. The newly emerging non-coding RNAs, so called microRNAs (miRNAs), are gaining tremendous attention as… Continue reading microRNA Delivery to Leukemic Cells