Transfection Reagent Selection Guide

The table below summarizes optimal transfection reagents for nucleic acids in different cell types.
The efficiency of the transfection reagents was assessed by using plasmid DNA (pDNA), short interfering RNA (siRNA), and messenger RNA (mRNA).
Where the transfection reagent was suitable for both pDNA or siRNA delivery, it was indicated with pDNA/siRNA.

Cell Type All-Fect Trans-Booster Leu-Fect-A Leu-Fect-B Prime-Fect mRNA-Fect CRISP-Fect
Primary Cells
Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) pDNA pDNA
mRNA
pDNA
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) pDNA pDNA
mRNA
pDNA
Vascular smooth muscle Cells (VSMCs) pDNA
mRNA
pDNA
Human Umbilical Vein Endothelial Cells (HUVECs) pDNA
mRNA
Mononuclear Cells from CML patients (MNC) pDNA
mRNA
siRNA siRNA
Human Foreskin Fibroblast Cells pDNA
Rat Primary Sympathetic Neurons pDNA mRNA
Cell Lines
Kidney Fibroblast Cells (293-T) pDNA pDNA
mRNA
pDNA
Breast Cancer Cells (MDA-MB-231) pDNA
siRNA
co-delivery
pDNA
mRNA
pDNA siRNA mRNA
Kidney Epithelial Cells (MDCK) siRNA
Breast Cancer/Melanoma Cells (MDA-MB-436) pDNA
mRNA
siRNA mRNA RNP
Breast Cancer Cells (MDA-MB-468) siRNA
Breast Cancer Cells (Sum-149PT) pDNA
mRNA
siRNA
Breast Cancer Cells (MCF-7) pDNA pDNA
mRNA
pDNA
siRNA
mRNA
Human Lymphoma Cells (U-937) pDNA pDNA
Chronic Myeloid Leukaemia Cells (K562) siRNA pDNA
mRNA
siRNA mRNA
Acute Myeloid Leukemia Cells (KG1 and KG1A) siRNA siRNA
Acute Myeloid Leukemia Cells (THP1) siRNA siRNA mRNA
Human Lung Cancer Cells (A549) siRNA
Human Colon Cancer (HCT-116) siRNA siRNA
Human Myoblasts ASO
Jurkat Cells pDNA mRNA RNP