Transfection Reagents

We developed broadly acting transfection reagents to modify mammalian cells with plasmid DNA, siRNA, mRNA and other nucleic acids.

 

The foundation of our transfection reagents is based on cationic lipopolymers with optimal balance of cationic charge and hydrophobic (lipid) group. By systematically altering the polymeric backbone and the nature of lipid group, a library of transfection reagents has been generated.

 

Some of the transfection reagents are broadly acting, functioning in different cell types with different nucleic acids. Others display high specificity, where they are exceptionally effective in particular cell types for particular nucleic acid delivery.

The key advantages of our delivery vehicles are:

 

  • Multivalent interactions with nucleic acids leading to strong binding of cargo that withstand disruptive forces in transit through cell membranes.
  • Synergetic effects due to cationic and lipidic binding that coat the cargo and protect it from nucleases.
  •  Lipidic moieties that enhance interactions with cell membrane and internalization.
  • pH buffering capacity that facilitate escape of cargo from endosomes.
  • Tailored formulations to free nucleic acids once internalized in cytoplasm. 
The transfection reagents have been optimized for the following cell models and applications:

Primary Cells

Attachment Dependent

  • VSMC (Vascular Smooth Muscle Cells)
  • Human Foreskin Fibroblast Cells
  • HUVEC (Human Umbilical Vein Endothelial Cells)
  • BMSC (Bone Marrow Stromal Cells)
  • Human Myoblasts

Attachment Independent

  • UCB-MSC (Umbilical Cord Blood Derived Mesenchymal Stem Cells)
  • BM-MSC (Bone Marrow Derived Mesenchymal Stem Cells)
  • Mononuclear Cells from Leukemia Patients
  • Mononuclear Cells from Normal Human Blood
  • Rat and Human Neurons

Cell Lines

Attachment Dependent

  • 293-T (Kidney Fibroblast Cells)
  • MDA-231, MDA-435, MDA-468, Sum-149PT, MCF-7 (Breast Cancer Cells)
  • A549 (Human Lung Cancer Cells)
  • MDCK (Kidney Epithelial Cells)
  • HCT-116 (Human Colon Cancer Cells)
  • C2C12 Myoblast Cells
  • MC3T3-1 (Preosteoblast Cells)
  • Green Monkey Vero Cells

Suspension-Growing

  • U-937 (Human Lymphoma Cells)
  • K562 (Chronic Myeloid Leukemia Cells)
  • KG1, KG1A, MV4-11 and THP-1 (Acute Myeloid Leukemia Cells)
  • Jurkat T-Cells
  • RS4;11 and SUP-B15 (Acute Lympocytic Leukemia Cells)

Animal Models

  • Systemic and local injection of RNAi mediator siRNA
  • Local injection of pDNA and mRNA expression vectors

For a classification our transfection reagents by cell type, please see our Transfection Reagent Selection Guide.

 

For a detailed description of our transfection reagents please see our summary of reagents page. Once you decide the right transfection reagent for your application, please proceed to our Store to place an order. We provide 30% discount for first-time customers; please use coupon code firsttime_30 in check-out.

 

Using a cell type or model that’s not on our list? We now offer screening services that can help find the best RJH reagent for your system

 

For questions about products and their use, please contact us at rjhbiosciences@gmail.com

Our Product Catalog

 

RJH manufactures a variety of transfection agents for your R&D needs. Download our catalogue for a list of our products.

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