siRNA, microRNA and ASO Delivery

US$148$295

  • Transient transfection with short oligonucleotides
  • Attachment dependent and independent cells
  • Stem, and primary cells
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Item Details
  • 30-10: Leu-Fect A reagent (0.75 mL at 1 mg/mL)
  • 30-20: Leu-Fect A reagent (1.5 mL at 1 mg/mL)
  • 40-10: Leu-Fect B reagent (1 mL at 5 mg/mL)
  • 40-20: Leu-Fect B reagent (1 mL at 5 mg/mL)
  • 10-10: ALL-Fect reagent (0.75 mL at 1 mg/mL)
  • 10-20: ALL-Fect reagent (1.5 mL at 1 mg/mL)
  • 20-10: Prime-Fect reagent (0.75 mL at 1 mg/mL)
  • 20-20: Prime-Fect reagent (1.5 mL at 1 mg/mL)

For more information about these reagents, please see Leu-Fect A, Leu-Fect B, ALL-Fect and Prime-Fect brochure .

siRNA/microRNA/ASO TransfectionTransfection reagents for siRNA/microRNA/ASO delivery in both in vitro and in vivo models.
Leu-Fect reagents are suitable for suspension-growing cells while ALL-Fect and Prime-Fect are suitable for attachment-dependent cells
Under some conditions, transfection kits containing Trans-Booster may help in delivery of the oligonucleotides
 SKUPack Size (mL)Concentration (mg/mL)Price (USD)
Leu-Fect A30-100.751.00154
30-201.501.00278
Leu-Fect B40-100.751.00154
40-201.501.00278
ALL-Fect10-100.751.00164
10-201.501.00295
Prime-Fect20-100.751.00148
20-201.501.00240
Benefits1. High transfection efficiency in the presence of serum.
2. Effective delivery of small RNA/DNA molecules via a simple protocol that is ideal for scale-up and automation.
3. Minimal toxicity compared to other commercial reagents, reducing adverse effects on highly sensitive human cells.
4. Non-integrating transfection reagent eliminates unwanted effects due to host genome integration.

 

Transfection Reagents for Delivery of Short Oligonucleotides

The indicated transfection reagents have been found to be highly effective in transient transfection of short RNA and DNA based oligonucleotides to a variety of cell types. Leu-Fect A and Leu-Fect B are ideal for modification of lymphocyte-like, suspension-growing cells. ALL-Fect and Prime-Fect are suitable for modification of attachment-dependent cells. In all cases, we recommend the users to inspect our Reagent Selection Guide (https://rjhbiosciences.com/transfection-reagent-selection-guide/) to determine the suitable reagent for the cell model to be used. All reagents are synthetic amphiphilic polymers that are selected for delivery of short oligos after extensive testing of amphiphilic polymer libraries. They are capable of undergoing multivalent interactions with oligonucleotides and encapsulating co-incubated oligos into ~100 nm particles with a net positive charge. The complexation between the oligos and the transfection reagents occurs in aqueous buffers, obviating the need for organic solvents during preparation. All reagents are non-integrating carriers, so that the genetic make-up of host cells is not altered after treatment with these reagents. ALL-Fect and Prime-Fect have been tested and found effective for plasmid DNA delivery as well as in certain cell types so that they can be used in combination delivery of large and small oligonucleotides. As with all transfection reagents, formulations of the reagents with oligonucleotides may need to be optimized for specific cell types and transfection conditions.

Overview

PropertyLeu-Fect A, Leu-Fect B, ALL-Fect and Prime-Fect
Nucleic AcidDNA and RNA Oligonucleotides
Cell TypesCommon, Stem, Primary, Suspension
Transfection TypeTransient, Co-delivery
High ThroughputCompatible
SerumCompatible
BufferAqueous
Storage Temperature-20ºC
Shelf Life at -20ºC~1 year

Transfections per 1 mL of Reagents

Plate Size Number of Transfections
6-well (1200 µL/well) 80 – 240
24-well (800 µL/well) 125 – 250
48-well (400 µL/well) 250 – 500
96-well (200 µL/well) 500 – 1000

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