Product Selection

Products for Delivery of Specific Types of Nucleic Acids
Transfection reagents from RJH Biosciences are optimized for delivery of a wide range of nucleic acids under in vitro (cell culture) and in vivo (animal models) conditions. For better efficiency with hard-to-transfect cell types and primary cells, we recommend using specific transfection kits that include the Trans-Booster reagent.
mRNA Delivery siRNA/microRNA/ASO Delivery
mRNA-Fect is specifically tailored for mRNA delivery to both attachment-dependent and suspension-growing cells. It is available as a stand-alone reagent as well as part of a kit with Trans-Booster. The kit is recomended for higher transfection efficiency in hard-to-transfect cells such as the suspension-growing primary cells. Leu-Fect A, Leu-Fect B, Prime-Fect and ALL-Fect are effective for delivery of small RNA compounds to a range of attachment-dependent and suspension-growing cells. ALL-Fect In Vivo and Leu-Fect reagents are recommended for use in animal models. Please consult the ‘Reagent Selection Guide’ (link) for finding the right reagent for your application.
DNA Delivery CRISPR Delivery
ALL-Fect is a versatile transfection reagent for pDNA delivery to a wide range of cells. ALL-Fect In Vivo is recommended for use in animal models while Prime-Fect is recommended for use on primary cells. The reagents are also available as a kit with Trans-Booster to maximize transfection efficiency. ALL-Fect and mRNA-Fect are versatile reagents to implement pDNA and mRNA based CRISPR protocols, respectively. CRISP-Fect is recommended for delivery of ribonucleoprotein (RNP) complexes to implement CRISPR. These reagents are tested for CRISPR under cell culture conditions only.

Transfection Reagent Selection Guide

The table below summarizes optimal transfection reagents for nucleic acids in different cell types. The efficiency of the transfection reagents was assessed by using plasmid DNA (pDNA), short interfering RNA (siRNA), and messenger RNA (mRNA). Where the transfection reagent was suitable for both pDNA or siRNA delivery, it was indicated with pDNA/siRNA.

Cell TypeAll-FectTrans-BoosterLeu-Fect-ALeu-Fect-BPrime-FectmRNA-FectCRISP-Fect
Primary Cells Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) pDNA pDNA mRNApDNA
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) pDNApDNA mRNApDNA
Vascular smooth muscle Cells (VSMCs)pDNA mRNApDNA
Human Umbilical Vein Endothelial Cells (HUVECs)pDNA mRNA
Mononuclear Cells from CML patients (MNC)pDNA mRNAsiRNAsiRNA
Human Foreskin Fibroblast CellspDNA
Rat Primary Sympathetic NeuronspDNAmRNA
Cell LinesKidney Fibroblast Cells (293-T)pDNApDNA mRNApDNA
Breast Cancer Cells (MDA-MB-231)pDNA siRNA co-deliverypDNA mRNApDNAsiRNAmRNA
Kidney Epithelial Cells (MDCK)siRNA
Breast Cancer/Melanoma Cells (MDA-MB-436)pDNA mRNA siRNA mRNARNP
Breast Cancer Cells (MDA-MB-468)siRNA
Breast Cancer Cells (Sum-149PT)pDNA mRNAsiRNA
Breast Cancer Cells (MCF-7)pDNApDNA mRNApDNA siRNAmRNA
Human Lymphoma Cells (U-937)pDNApDNA
Chronic Myeloid Leukaemia Cells (K562)siRNApDNA mRNAsiRNAmRNA
Acute Myeloid Leukemia Cells (KG1 and KG1A)siRNAsiRNA
Acute Myeloid Leukemia Cells (THP1)siRNAsiRNAmRNA
Human Lung Cancer Cells (A549)siRNA
Human Colon Cancer (HCT-116)siRNAsiRNA
Human MyoblastsASO
Jurkat CellspDNAmRNARNP