pDNA Transfection Reagents

US$254$2,100

  • For pDNA administration in Cell lines, Prime cells and animal models
  • Cost-effective for in vivo applications, with improved transfection efficiency and minimal toxicity
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Item Details

The DNA transfection reagents can be ordered in several configurations depending on application needs:

  • 10-10: ALL-Fect reagent (0.75 mL at 1 mg/mL)
  • 10-20: ALL-Fect reagent (1.5 mL at 1 mg/mL)
  • 10-30: ALL-Fect In Vivo reagent (1 mL at 5 mg/mL)
  • 10-40: ALL-Fect Kit (0.75 mL ALL-Fect reagent at 1 mg/mL+ 0.75 mL Trans-Booster at 0.4 mg/mL)
  • 10-50: ALL-Fect kit (1.5 mL ALL-Fect reagent at 1 mg/mL + 1.5 mL Trans-Booster at 0.4 mg/mL)
  • 10-60: ALL-Fect In Vivo Kit (1 mL ALL-Fect reagent at 5 mg/mL + 1 mL Trans-Booster at 2 mg/mL)
  • 20-40: Prime-Fect In Vivo kit (0.75 mL Prime-Fect reagent at 1 mg/mL+ 0.75 mL Trans-Booster at 0.4 mg/mL)
  • 20-50: Prime-Fect In Vivo kit (1.5 mL Prime-Fect reagent at 1 mg/mL+ 1.5 mL Trans-Booster at 0.4 mg/mL)
 
For more information about the DNA transfection reagents, please see the ALL-Fect and Prime-Fect brochures.
DNA Transfection ALL-Fect and Prime-Fect transfection reagents for pDNA delivery in both in vitro and in vivo models.
ALL-Fect Kit is recommended for better efficacy in hard-to-transfect cell types
ALL-Fect Kit contains 1 vial of ALL-Fect and 1 vial of Trans-Booster
SKU Pack Size (mL) Concentration (mg/mL) Price (USD)
ALL-Fect 10-10 0.75 1.00 265
10-20 1.50 1.00 480
ALL-Fect In Vivo 10-30 1.00 5.00 1500
ALL-Fect Kit 10-40 0.75 / 0.75 1.00 371
10-50 1.5 / 1.5 1.00 672
ALL-Fect In Vivo Kit 10-60 1.0 / 1.0 5.0 / 2.0 2100
Prime-Fect In Vivo Kit 20-40 0.75 / 0.75 1.0 / 0.4 254
20-50 1.5 / 1.5 1.0 / 0.4 432
Benefits 1. High transfection efficiency in the presence of serum.
2. Effective delivery of pDNA molecules via a simple protocol that is ideal for scale-up and automation.
3. Minimal toxicity compared to other commercial reagents, reducing adverse effects on highly sensitive human cells.
4. Non-integrating transfection reagent eliminates unwanted effects due to host genome integration.
5. Possibility of using the same transfection reagent in animal models, leading to harmonized studies.

DNA Transfection Reagents

ALL-Fect and Primet-Fect are highly effective delivery agents for use in animal studies. They are synthetic amphiphilic polymers that are tailored for plasmid DNA delivery. They are capable of undergoing multivalent interactions with DNA and encapsulating co-incubated DNA molecules into 100-150 nm particles with a net positive charge. The complexation between the plasmid DNA and the transfection reagents occur in aqueous buffers, obviating the need for organic solvents during preparation. The transfection reagents are non-integrating carriers of DNA, so that the genetic make-up of host cells is not altered after treatment with the delivery reagent. ALL-Fect and Prime-Fect has been tested and found effective in different types of attachment dependent cells as well for cell culture use, but users are advised to test the efficacy of the reagent with their particular cell type to confirm utility in specific applications. As with all delivery agents, formulations of the transfection reagents with pDNA may need to be optimized for specific applications in vivo.

Overview

PropertyALL-Fect and Prime-Fect
Nucleic AcidpDNA
Transfection TypeTransient, In Vivo
High ThroughputCompatible
BufferAqueous
Storage Temperature-20ºC
Shelf Life at -20ºC1 year

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