mRNA Transfection Reagents

US$265$2,100

  • Attachment dependents such as MCF-7, MDA-MB-436, MDA-MB-231 cells, and suspension cell lines such as K562 and THP-1 cells
  • Optimized for mRNA transfection in transient protein expression
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Item Details

The mRNA-Fect can be ordered in several configurations depending on application needs:

  • 80-10: mRNA-Fect reagent (0.75 mL at 1 mg/mL)
  • 80-20: mRNA-Fect reagent (1.5 mL at 1 mg/mL)
  • 80-30: mRNA-Fect reagent (1 mL at 5 mg/mL)
  • 80-40: mRNA-Fect kit (0.75 mL mRNA-Fect reagent at 1 mg/mL+ 0.75 mL Trans-Booster at 0.4 mg/mL)
  • 80-50: mRNA-Fect kit (1.5 mL mRNA-Fect reagent at 1 mg/mL + 1.5 mL Trans-Booster at 0.4 mg/mL)
  • 80-60: mRNA-Fect In Vivo kit (1 mL mRNA-Fect reagent at 5 mg/mL + 1 mL Trans-Booster at 2 mg/mL)

For more information about the mRNA-Fect reagent, please see the product brochure

mRNA TransfectionTransfection reagents for mRNA delivery in both in vitro and in vivo models.
mRNA-Fect Kit is recommended for better efficacy in hard-to-transfect cell types
mRNA-Fect Kit contains 1 vial of mRNA-Fect and 1 vial of Trans-Booster
     SKUPack Size (mL)Concentration (mg/mL)Price (USD)
mRNA-Fect80-100.751.00265
80-201.501.00480
mRNA-Fect In Vivo80-301.005.001500
mRNA-Fect Kit80-400.751.00371
80-501.501.00672
mRNA-Fect In Vivo Kit80-601.0 / 1.05.0 / 2.02100
Benefits1. High transfection efficiency in the presence of serum.
2. Effective delivery of mRNA molecules via a simple protocol that is ideal for scale-up and automation.
3. Minimal toxicity compared to other commercial reagents, minimally affecting highly sensitive human cells.
4. Non-integrating transfection reagent eliminates adverse effects due to host genome integration.
5. Possibility of using the same transfection reagent in animal models, leading to harmonized studies.
6. Cost-effective reagent minimizing additional costs in nucleic acid screens.
mRNA-Fect: mRNA Transfection Reagent

mRNA-Fect is a highly effective transient transfection reagent optimized for mRNA delivery to both attachment-depended and suspension-growing cells. mRNA-Fect is a synthetic amphiphilic polymer that is tailored for mRNA delivery after extensive testing of amphiphilic polymer libraries. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated mRNA into ~100 nm particles with a net positive charge. The complexation between the mRNA and mRNA-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. mRNA-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with this reagent. mRNA-Fect has been tested and found effective for plasmid DNA and siRNA delivery as well as in certain cell types. As with all transfection reagents, formulations of mRNA-Fect with polynucleotides may need to be optimized for specific cell types and transfection conditions.

Overview
PropertymRNA-Fect
TechnologyPolymeric
Nucleic AcidmRNA
Cell TypesSuspension, Attachment
Transfection TypeTransient
High ThroughputCompatible
SerumCompatible
BufferAqueous
Storage Temperature-20 ºC
Shelf Life at -20 ºC~1 year
Transfections per 1 mL of mRNA-Fect
Plate SizeNumber of Transfections
6-well (1200 µL/well)40 – 330
24-well (800 µL/well)60 – 500
48-well (400 µL/well)125 – 1000
96-well (200 µL/well)250 – 2000

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