At RJH Biosciences, we design polymer-based nucleic acid delivery platforms to enable high-efficiency gene editing in primary and hard-to-transfect cell lines. CRISP-Fect provides a robust, non-viral platform for Cas9 ribonucleoprotein (RNP) delivery and gene editing.
Robust Phenotypic Knockout
CRISPR–Cas9 complexes delivered with CRISP-Fect achieved potent target gene disruption in MDA-MB-231 GFP cells. Quantitative fluorescence analysis demonstrated ~70% reduction in GFP signal relative to control sgRNA.
Sequence-Level Validation
Inference of CRISPR Edits (ICE) analysis of Sanger sequencing data verified precise double-strand break repair at the genome. CRISP-Fect-mediated Cas9 delivery yielded 55% indel efficiency.
Functional Therapeutic Knockout
CRISP-Fect was utilized to deliver Cas9 RNP complexes targeting the essential survival gene Survivin (Survivin KO sgRNA) in MDA-MB-231 wild-type cells. Targeted gene knockout resulted in a ~65% reduction in cell viability.
About RJH Biosciences
RJH Biosciences specializes in lipopolymer-based transfection reagents for efficient sgRNA, siRNA, mRNA, and DNA delivery across a wide range of biological systems. Our platforms are optimized for high-throughput screening, functional genomics, and RNA interference (RNAi) applications, enabling reliable gene modulation in cell lines, primary cells, and in vivo models.
We are Here to Help
Learn more about CRISP-Fect
https://rjhbiosciences.com/product/crisp-fect-rnp-transfection-reagent/
Get personalized advice from RJH on the best transfection info@rjhbiosciences.com or visit www.rjhbiosciences.com
