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Delivery of Nucleic Acids with RJH Biosciences Reagents
RJH Biosciences facilitates delivery of nucleic acids to biological systems, including siRNA, mRNA, pDNA, miR and ASO. Our polymeric reagents provide stable and effective delivery of polynucleotides in various cell types and animal models. RJH reagents allow your studies in cell culture and animal models to be executed in a way that is safe and reliable. We are dedicated to:
Implementing CRISPR-Cas9 Technology using RJH Reagents
CRISPR technologies have been increasingly utilized in research and therapeutic development. RJH has been collaborating with leading CRISPR experts to validate our reagents for CRISPR applications. The following shows Prime-Fect and other RJH reagents used for gene editing using pDNA-based CRISPR-Cas9 technology.
Figure. Top: Endonuclease assays of WTAP and RunX editing using CRISPR-Cas9. Transfection of the cells was performed with Prime-Fect and Lipo3000. Multiple DNA bands indicate positive DNA cleavage by the Cas9 endonuclease. Bottom: Relative transfection efficiencies of CRISPR-Cas9 plasmid by a leading lipofection product as compared to four RJH reagent formulations.
Our reagents provide high transfection efficiencies as compared to leading market reagents that lead to strong gene editing. A full testimonial on RJH reagents in CRISPR applications can be found here. Contact us if you are interested in using our reagents for plasmid and protein based (RNP) CRISPR technology.
To try out Prime-Fect for your transfection experiments click on the link below:
News Release
We have recently announced collaborations with CasZyme LLC and Kernal Biologics in our latest news release! Check out the article here.
All your transfection needs, provided by us!
In addition to providing transfection reagents, we also offer a screening service and other resources to identify optimal transfection reagents for polynucleotides of your choice. Our in-house screening services covers up to a 1000 reagents and formulations. We can help you optimize your transfection conditions and ensure long term success.
An application form for our screening services is on our website (click here).
We are happy to help!
Get personalized advice by an RJH representative on the best transfection methods for a specific application. We look forward to working with you soon!
RJH manufactures a variety of transfection agents for your R&D needs. Download our catalogue for a list of our products.