CRISPR Transfection Reagents

The indicated transfection reagents have been found to be highly effective in transient transfection of reagents necessary for implementing CRISPR. All reagents are ideal for implementing CRISPR in attachment-dependent cells based on specific type of nucleic acid to be used for editing. We recommend the users to inspect our Reagent Selection Guide (https://rjhbiosciences.com/transfection-reagent-selection-guide/) to determine the suitable reagent for the cell model to be used. All reagents are synthetic amphiphilic polymers that are validated for delivery of indicated CRISPR reagents after extensive testing of amphiphilic polymer libraries. They are capable of undergoing multivalent interactions with oligonucleotides and encapsulating co-incubated nucleic acids into ~100 nm particles with a net positive charge. The complexation between the nucleic acids and the transfection reagents occurs in aqueous buffers, obviating the need for organic solvents during preparation. All reagents are non-integrating carriers, so that the genetic make-up of host cells is not altered after treatment with these reagents. The transfection kits are suitable for hard to transfer cell types while the use of individual reagents was found effective in easily transfectable cell types. As with all transfection reagents, formulations of the reagents with oligonucleotides may need to be optimized for specific cell types and transfection conditions.