Month: March 2022

Killing Lung Cancer A549 Cells with RJH Reagents and Cytotoxic siRNAs Testimonial: mRNA Killing Lung Cancer A549 Cells with RJH Reagents and Cytotoxic siRNAs Background Lung cancer is a deadly disease. Cancers originating in lungs or metastasized to lungs from other sites are particularly difficult to treat due to several mechanisms that facilitate cancer cells… Continue reading Killing Lung Cancer A549 Cells with RJH Reagents and Cytotoxic siRNAs

mRNA Transfection of Endothelial Cells with RJH Reagents Testimonial: mRNA Transfection of Endothelial Cells with RJH Reagents Background Delivering messenger RNA (mRNA) is a new approach for rapid expression and secretion of proteins in human cells. It is providing a more effective alternative to plasmid DNA based expression systems since it may not be necessary… Continue reading mRNA Transfection of Endothelial Cells with RJH Reagents

Transfecting Human Astrocytes with RJH Reagents: GFP Expression and siRNA Uptake Testimonial: Transfecting Human Astrocytes with RJH Reagents: GFP Expression and siRNA Uptake Background Astrocytes is an important cell type that participates in hemostasis of brain tissue. They perform several functions, including biochemical support of endothelial cells that form the blood brain barrier, nutrient support… Continue reading Transfecting Human Astrocytes with RJH Reagents:
GFP Expression and siRNA Uptake

Transfecting Colon Cancer HCT-116 Cells with RJH Reagents to silence Polynucleotide Kinase 3′-Phosphatase (PNKP) Expression Testimonial: Transfection of A549 Lung Cancer Cells with RJH Reagents to Silence Tumor Suppressor p53 ExpressionMethods: Lung cancer A549 cells (ATCC) were seeded in 6-well plates with 500 µL of tissue culture medium without antibiotics (DMEM + 10% FBS) for… Continue reading Transfection of A549 Lung Cancer Cells

Transfecting Colon Cancer HCT-116 Cells with RJH Reagents to silence Polynucleotide Kinase 3′-Phosphatase (PNKP) Expression Testimonial: Transfecting Colon Cancer HCT-116 Cells with RJH Reagents to silence Polynucleotide Kinase 3′-Phosphatase (PNKP) Expression Methodology Colon cancer HCT-116 cells (ATCC) were seeded and grown in 6-well plates overnight before transfection with siRNA complexes. A specific siRNA against Polynucleotide… Continue reading Transfecting Colon Cancer HCT-116 Cells with RJH Reagents to silence Polynucleotide Kinase 3′-Phosphatase (PNKP) Expression

Use of RJH Transfection Reagents in Antisense Oligonucleotide Aso Delivery Testimonial: Use of RJH Transfection Reagents in Antisense Oligonucleotide Delivery Background The convergences of sophisticated delivery systems, oligonucleotide chemistry and genetic bioinformatics has significantly enhanced the exploration of antisense oligonucleotides for therapeutic purposes, which has been reflected in many clinical studies (Nat. Rev. Drug Discov.… Continue reading Use of RJH Transfection Reagents in Antisense Oligonucleotide Aso Delivery

Transfecting Triple Negative Testimonial: Transfecting Triple-Negative Breast Cancer MD-MB-231 Cells with Plasmid DNA and siRNA using All-Fect and Prime-Fect. Western Blot analysis of breast cancer cells transfected with siRNA/Prime-Fect complexes  A specific siRNA against LPP1 (Lipid Phosphate Phosphohydrolase Type I) was formulated with Prime-Fect and added to the cells at 60 and 120 pmol (cells… Continue reading Transfecting Triple Negative

Implementing CRISPR-Cas9 Technology using Transfection Reagents from RJH Biosciences Testimonial: Implementing CRISPR-Cas9 Technology using Transfection Reagents from RJH Biosciences Background Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a leading gene editing tools widely used today. The CRISPR-Cas9 system can effectively and accurately target DNA sequences with the use of an RNA scaffold, known as… Continue reading Implementing CRISPR-Cas9 Technology using Transfection Reagents from RJH Biosciences