Prime-Fect: Primary Cell Transfection Reagent


  • Optimized for primary cells
  • pDNA transient transfection
  • Mesenchymal stem cells, and highly differentiated cells
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Prime-Fect: Designed for Primary Cell Transfection

Prime-Fect has been crafted as a highly effective reagent for primary cell transfection plasmid DNA. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the Prime-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. As a non-integrating carrier, the genetic make-up of host cells is not altered after treatment with the transfection reagent. Prime-Fect has also been tested and found effective in different types of attachment dependent cell lines for siRNA delivery. As with all transfection reagents, formulations of Prime-Fect with nucleic acid may need to be optimized for specific cell types and transfection conditions.  Learn More


Nucleic AcidpDNA, siRNA
Cell TypesPrimary (pDNA), and other cell lines (siRNA)
Transfection TypeTransient
High ThroughputCompatible
Storage Temperature4ºC
Shelf Life at -20ºC~1 year


Transfections per 1  mL of Prime-Fect:

Plate SizeNumber of Transfections
6-well (1200 µL/well)80 – 240
24-well (800 µL/well)80 – 200
48-well (400 µL/well)160 – 410
96-well (200 µL/well)330 – 830


Validated Cell Types:

Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC)Breast Cancer Cells (MDA-231, MDA-435, MDA-468, SUM-149PT, MCF-7)
Bone Marrow Derived Mesenchymal Steam Cells (BM-MSC)Kidney Epithelial Cells (MDCK)
Human Lymphoma Cells (U-937)Human Lymphoma Cells (U-937)