Leu-Fect A & B: Leukaemia Cell Transfection Reagents

Leu-Fect A and B are a highly effective transfection reagent for attachment independent (suspension-growing) cells. Leu-Fect A and B were originally designed as leukaemia cell transfection reagents but, have proven effective on other suspension growing cells and adherent cells. Leu-Fect is a synthetic amphiphilic polymer that was specifically tailored for siRNA delivery to leukaemia cells. It is capable of undergoing multivalent interactions with siRNA and encapsulating co-incubated siRNA molecules into 100 nm particles with a net positive charge. The complexation between the siRNA and Leu-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. Leu-Fect A and B are a non-integrating carrier of siRNA, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent. Leu-Fect has been tested and found effective in different types of attachment-independent cells, but users are advised to test the efficacy of the reagent in their particular cell type in order to choose the right formulation for long-term use. As with all transfection reagents, formulation of Leu-Fect with siRNA may need to be optimized for specific cell types.

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