US$154 – $276
Leu-Fect A and B are a highly effective transfection reagent for attachment independent (suspension-growing) cells. Leu-Fect A and B were originally designed as leukaemia cell transfection reagents but, have proven effective on other suspension growing cells and adherent cells. Leu-Fect is a synthetic amphiphilic polymer that was specifically tailored for siRNA delivery to leukaemia cells. It is capable of undergoing multivalent interactions with siRNA and encapsulating co-incubated siRNA molecules into 100 nm particles with a net positive charge. The complexation between the siRNA and Leu-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. Leu-Fect A and B are a non-integrating carrier of siRNA, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent. Leu-Fect has been tested and found effective in different types of attachment-independent cells, but users are advised to test the efficacy of the reagent in their particular cell type in order to choose the right formulation for long-term use. As with all transfection reagents, formulation of Leu-Fect with siRNA may need to be optimized for specific cell types.
Property | Leu-Fect A | Leu-Fect B |
Nucleic Acid | siRNA | siRNA |
Cell Types | Suspension-growing | Suspension-growing |
Transfection Type | Transient | Transient |
High Throughput | Compatible | Compatible |
Serum | Compatible | Compatible |
Buffer | Aqueous | Aqueous |
Storage Temperature | -20 ºC | 4 ºC |
Shelf Life at -20 ºC | 1 year | 1 year |
Plate Size | Number of Transfections |
---|---|
6-well (1200 µL/well) | 80 – 240 |
24-well (800 µL/well) | 125 – 250 |
48-well (400 µL/well) | 250 – 500 |
96-well (200 µL/well) | 500 – 1000 |
Leu-Fect A (siRNA Delivery) | Leu-Fect B (siRNA Delivery) |
---|---|
Chronic myeloid leukaemia cells (K562) | Acute myeloid leukaemia cells (THP1) |
Mononuclear Cells (MNC) from CML patients | Mononuclear Cells (MNC) from CML patients |
RJH manufactures a variety of transfection agents for your R&D needs. Download our catalogue for a list of our products.